Surface plasmon resonance biosensors: advances, challenges and applications
Jiří Homola
Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic
17:00 - 18:00 Tuesday 22 June 2010 KFU HS 05.01 There has been a tremendous effort towards the development of biosensor technologies for the detection and identification of chemical and biological species. Label-free optical biosensors represent a unique technology that enables direct observation of molecular interaction in real-time and offers benefits of rapid, sensitive and label-free detection of chemical and biological species with potential applications in numerous important areas. Optical biosensors based on surface plasmon resonance (SPR) represent the most advanced and mature optical label-free biosensor technology.
Over the last two decades optical biosensors based on surface plasmon resonance (SPR) have become important tool for real-time study of biomolecular interactions and for detection of analytes in fields such as food safety, medical diagnostics, or environmental monitoring [1]. This lecture reviews the present state of the art in the development of SPR sensor technology, discusses emerging trends, and presents selected results of research into SPR sensors at the Institute of Photonics and Electronics, Prague. The advances to be discussed in detail include multiple-plasmon spectroscopy for bioanalysis of complex samples, polarization-contrast SPR imaging for high-throughput SPR biosensing and a new approach to spectroscopy of surface plasmons allowing construction of compact low-cost SPR sensing devices. In addition, applications of SPR biosensors will be reviewed and selected examples of SPR biosensors for detection of analytes related to medical diagnostics, environmental monitoring, food safety and security will be presented [2].
[1] J. Homola, Surface plasmon resonance based sensors, Springer (2006).
[2] J. Homola, Chem. Rev., 108, p. 462 (2008).
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